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The vectors feline immunodeficiency virus and adenovirus transfect different cell types in rat nucleus tractus solitarii
Author(s) -
Lin LiHsien,
Corr Julie,
Talman William T.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a466-a
Subject(s) - neun , feline immunodeficiency virus , transduction (biophysics) , biology , microbiology and biotechnology , glial fibrillary acidic protein , transfection , viral vector , green fluorescent protein , astrocyte , immunostaining , cell culture , virology , lentivirus , virus , immunology , gene , central nervous system , immunohistochemistry , recombinant dna , neuroscience , genetics , viral disease , biochemistry
Gene transfer has been used to examine the role of putative neurotransmitters in the nucleus tractus solitarii (NTS), a brain stem nucleus that modulates diverse autonomic functions. Most such studies used adenovirus (Ad)‐mediated gene transfer with cytomegalovirus promoter. Other studies showed that transduction with Ad occurred mainly in non‐neuronal cells. In contrast, feline immunodeficiency virus (FIV) may preferentially transfect neurons. We sought to determine if the two vectors transduced protein expression in the same or different cell types in the NTS. Using double fluorescent immunostaining combined with confocal microscopy, we examined expression of the reporter gene LacZ 3‐4 days after introducing Ad‐LacZ or FIV‐LacZ into the NTS. Our results showed that the majority of NTS cells expressing β galactosidase (βGal)‐immunoreactivity (IR) after microinjection of Ad‐LacZ into the NTS contained the glial marker glial fibrillary acidic protein (GFAP)‐IR, but not the neuronal marker neuronal nuclear antigen (NeuN)‐IR. In contrast, after FIV‐LacZ injection, all βGal‐IR expressing NTS cells contained NeuN‐IR, but not GFAP‐IR. We conclude that Ad transduced mainly glial cells, while FIV transduced mainly neurons in the NTS. The cell‐type specificity of Ad and FIV in the NTS may provide a unique means for dissecting molecular mechanisms of NTS functions. Support: NIH HLR01 59593 and VA Merit Review.