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Nucleus tractus solitarii (NTS) expression of GAD67 and neurokinin receptors (NK1‐R) in transgenic GAD67‐eGFP mice
Author(s) -
McGovern David,
Fong Angelina Y,
Potts Jeff T
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a465-b
Subject(s) - glutamate decarboxylase , solitary nucleus , biology , microbiology and biotechnology , receptor , chemistry , nucleus , endocrinology , medicine , enzyme , biochemistry
Glutamic acid decarboxylase (GAD67) and NK1‐R are important components of autonomic circuits controlling cardiorespiratory function. The purpose of this study was to determine the distribution of GAD67 neurons in the NTS using transgenic mice expressing enhanced green fluorescent protein (eGFP) controlled by the GAD67 promoter. Adult mice (n=2) were anesthetized and transcardially‐perfused. Cryostat sections (30μm) containing the NTS were stained with a polyclonal antibody against NK1‐Rs and visualized with Cy3 secondary antibody. GAD67 and NK1‐R expressing cells were counted and sections reconstructed using Neurolucida software. A total of 15,404 medullary neurons were counted. Of the total cells, 4,767±756 were GAD67 cells and 2,830±941 were NK1‐R positive, while 106±31 were double‐labeled. Within the NTS, 822±152 were GAD67 positive, 335±83 expressed NK1‐Rs and only 7±4 were double‐labeled. At caudal levels of the NTS (Bregma −14.3 to −13.1), GAD67 cells were predominantly clustered around the solitary tract, while at more rostral levels (Bregma −13.24) they were localized to the medial and ventrolateral subdivisions. GAD67 labeling in mice was different than the pattern recently reported in rats (Stornetta & Guyenet, 1999; Fong et al., 2005). This may suggest a difference in inhibitory modulation of afferent signals by the NTS. Supported by NIH HL059167 (JTP).