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High‐fat feeding decreases C‐reactive protein (CRP) in rats
Author(s) -
Shin Andrew Changhun,
MohanKumar Sheba MJ,
Fink Gregory,
Haywood Joseph R,
MohanKumar Puliyur
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a458-a
Subject(s) - medicine , endocrinology , c reactive protein , obesity , leptin , inflammation
Diet‐induced obesity (DIO) is a serious health problem. This condition is associated with dysregulation of the stress axis, which could probably contribute to obesity as well. Using DIO and diet resistant (DR) rat models, we have shown that feeding a high‐fat (HF) diet causes leptin levels to increase only in DIO animals. However, DR animals have activated stress axis after HF diet exposure. We hypothesized that in DR rats, HF diet can trigger a stress‐like response by stimulating the production of C‐reactive protein (CRP), an inflammatory marker produced by the liver. This could contribute to increased stress axis activity. To test this, DIO and DR rats were fed a HF diet or chow for six weeks. Trunk blood was collected upon sacrifice for quantification of CRP by ELISA (Helica Biosystems, Inc. CA). CRP levels (Mean±S.E.; μg/ml) were highest in chow‐fed DR animals (68.48±6.6) followed by chow‐fed DIO rats (52.44±7.6). Exposure to a HF diet decreased CRP levels significantly to 19.57±1.8 in DIO rats and to 31.28±4.7 in DR rats (p<0.01). The significance of this decrease in CRP levels after HF diet exposure is not clear. The dynamics of CRP production during different stages of HF diet exposure may help us understand the role of CRP in this phenomenon. Supported by NIH AG 027697 and Biomedical Health Research Initiative, MSU.