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Emerging data from the PABS study: using isotope coded affinity tags (ITRAQ) for longitudinal Proteomic Analysis following a weight loss intervention, Bariatric Surgery, in morbidly‐obese subjects
Author(s) -
Lynch Christopher J,
Stanley Bruce A,
Stanley Anne E,
Eicher Rachel M,
Cooney Robert C
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a457-c
Subject(s) - adipose tissue , weight loss , proteomics , biomarker , medicine , obesity , gastric bypass surgery , diabetes mellitus , bioinformatics , gastroenterology , chemistry , surgery , endocrinology , biochemistry , biology , gastric bypass , gene
Morbid obesity is associated with multiple medical conditions including: type 2 diabetes, sleep apnea, hypertension, GERD and others. The cause of these obesity‐related diseases and how they are improved following gastric bypass surgery (GBP) remains unknown. Discovery proteomics is being used to address this question. Candidates for GBP surgery have been recruited since 2003 for tissue banking. A subset return for another surgical procedure (e.g., panniculectomy, hernia repair, or gallbladder removal) following weight loss when all or some obesity co‐morbidities have improved. This provides an opportunity for longitudinal P roteomic A nalysis of B ariatric S urgery (PABS). We have blood, plasma, skeletal muscle and adipose tissue depots (freeze clamped in OR) from over 120 subjects, with ~20 returns so far. Adipocytes from omental (visceral) and subcutaneous fat are also banked. For serum or tissue lysates, depletion columns are used to remove major plasma proteins. Alternatively, subcellular fractions are analyzed. Tryptic peptides from these complex samples are labeled with iTRAQ reagent (an isobaric, multiplexable isotope coded affinity tag quantifiable by mass spectrometry in TOF‐TOF mode), decomplexed by 2D liquid chromatography, analyzed by mass spectrometry, leading to ID and relative quantification of ~1000 proteins per run. Emerging results and candidate biomarker proteins will be presented. PA‐DOH