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Effects of ganoderan on cytochrome C and mitochondrial calcium in brain tissues from epilepsy rats
Author(s) -
Wang ShuQiu,
Kang* YuMing,
Qin WenBo,
Lu ChunFeng,
Wang ShuXiang,
Zhang ShiLing,
Chen TingYu,
Ma XiaoRu,
Liang YanFeng,
Wang FangFang
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a451-a
Subject(s) - epilepsy , malondialdehyde , superoxide dismutase , cytochrome c , pharmacology , medicine , chemistry , endocrinology , apoptosis , anesthesia , biochemistry , oxidative stress , psychiatry
The present study was undertaken to observe the effects of ganoderan on mitochondrial Ca 2+ , cytochrome C, and cytosolic malondialdehyde (MDA), superoxide dismutase (SOD) in brain tissues from epilepsy rats. Methods: Epilepsy rats were made by intraperitoneal injection of sub‐convulsive dosage of pentetrazole (PTZ, 32mg/kg/day for 21 days). Twenty epilepsy rats were divided into ganoderan‐treated epilepsy group and vehicle‐treated epilepsy group, and 10 normal rats were used as control. Flame atomic absorption spectrometry and spectrophotometric analysis were used to detect levels of mitochondrial Ca 2+ , SOD, MDA, and cytochrome C in brain tissues. Results: After treatment with ganoderan (by gastric gavage) for 28 days, the levels of mitochondrial Ca 2+ (2.253±0.104 vs 2.132±0.115 mg/L) and cytochrome C (1.471±0.120 vs 1.168±0.095 mmol/L) were higher (ganoderan‐treated epilepsy group vs vehicle‐treated epilepsy group, P<0.05), and the levels of cytosolic cytochrome C (7.98±0.48 vs 8.67±0.61 mmol/L) and MDA (102.21±11.67 vs 105.29±18.99 mg/L) were lower (ganoderan‐treated epilepsy group vs vehicle‐treated epilepsy group, P<0.05), and the level of SOD (2301.04±155.24 vs 1863.27±186.68 U/mg protein) was higher (ganoderan‐treated epilepsy group vs vehicle‐treated epilepsy group, P<0.05) in the ganoderan‐treated epilepsy rats than the vehicle‐treated epilepsy rats. Conclusion: Ganoderan exerts protective effects on brain cell mitochondria in epilepsy by clearing free radical and strengthening antioxidation of brain tissues.