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Superoxide anion mediates Ang II‐induced ET‐1 expression in mouse adventitial fibroblasts
Author(s) -
Chapman Sandy,
An Shengjun,
Di Wang Hui
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a450-c
Subject(s) - superoxide , angiotensin ii , chemistry , messenger rna , procollagen peptidase , endothelin 1 , microbiology and biotechnology , superoxide dismutase , western blot , tiron , fibroblast , renin–angiotensin system , endocrinology , medicine , in vitro , biology , biochemistry , enzyme , receptor , gene , blood pressure
Our objective was to determine if superoxide anion mediates endothelin‐1 (ET‐1) expression in adventitial fibroblasts in response to angiotensin II (Ang II). Vascular adventitial fibroblasts were isolated from mouse aorta, preproET‐1 messenger RNA (mRNA) levels were determined by relative RT‐PCR and procollagen protein was measured by Western blot. Adventitial fibroblasts were treated with or without Ang II (10 −7 M) and antioxidants superoxide dismutase (SOD) (350 U/ml), tempol (10 −5 M), and tiron (10 −5 M). Ang II significantly increased preproET‐1 mRNA and procollagen expression while treatment with antioxidants decreased both significantly (p<0.05). To further examine the role of superoxide, Cu 2+ /Zn 2+ SOD was overexpressed in the adventitial fibroblasts and decreased levels of preproET‐1 mRNA and procollagen were found with Ang II treatment when compared to control (p<0.05). In conclusion, Ang II is able to increase both superoxide and ET‐1 production and inhibition of superoxide leads to a decrease in ET‐1 expression demonstrating that superoxide mediates Ang II induced ET‐1 expression in mice adventitial fibroblasts. Funding provided by the Canadian Institute of Health Research.