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EFFECTS OF AGING AND EXERCISE ON CYSTATHIONINE‐γ‐LYASE EXPRESSION IN MOUSE GASTROCNEMIUS MUSCLE
Author(s) -
Wisniewski Nicole H,
Chaudhari Kritika,
Bearden Shawn E
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a448-a
Subject(s) - oxidative stress , gastrocnemius muscle , medicine , endocrinology , cystathionine gamma lyase , sed , downregulation and upregulation , cystathionine beta synthase , chemistry , treadmill , cysteine , skeletal muscle , biochemistry , enzyme , gene
Oxidative stress increases with aging and exercise. It has been suggested that the production of hydrogen sulfide (H 2 S) from cysteine via cystathionine‐γ‐lyase (CSE) is upregulated under conditions of oxidative stress. We tested the hypothesis that exercise training and aging would increase CSE protein expression. The gastrocnemius muscle was collected from young (4–5 mo), adult (12–14 mo) and old (19–21 mo) male C57BL6 mice that were housed in either sedentary (no running wheel) or exercise trained (access to a voluntary running wheel) conditions for eight weeks (n=3/group), and the muscle tissues were analyzed for CSE protein expression. The optical density of the anti‐mouse CSE labeled protein bands on Western blots were significantly different among all age group comparisons for sedentary mice (p<0.05): young (74.6 ± 0.8 AU), adult (80.7 ± 1.5 AU) and old (92.5 ± 1.2 AU). Exercise training increased CSE expression (p<0.05) in young (91.5 ± 4.2 AU) and adult (95.6 ± 0.4 AU), but not in old (94.7 ± 3.7 AU; p>0.05) mice compared to sedentary mice. These studies uniquely demonstrate that increased expression of CSE, which produces H 2 S, is a common adaptation to the stresses of aging and exercise.