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Toxicological evaluation of amphotericin B and its synthetic analogue in mice
Author(s) -
Buitimea Angel León,
Nava Rosmarbel Morales,
Zertuche Mario Fernández,
Blake Iván Ortega,
ReyesEsparza Jorge,
RodríguezFragoso Lourdes
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a441
Subject(s) - amphotericin b , nephrotoxicity , pharmacology , toxicity , medicine , apoptosis , bone marrow , dose , necrosis , chemistry , immunology , biochemistry , antifungal , dermatology
At present there are not effective drugs for fungal infections. The amphotericin B has been used during 45 years. However, dose dependent nephrotoxicity has been a persistent problem, often limiting its administration in full therapeutic dosages. An alternative approach for limiting amphotericin nephrotoxicity has been to synthesize new analogous. The aim of this work was to evaluate and compare the toxicity of and analog of amphotericin B in BalB‐c mice. For the study we used male mice 25g whom received 4 mg/kg of analogous and amphotericin B by i.p., during 10 days in unique dosage every third day. After treatment animals were sacrificed and were analyzed the renal function and was done and histological and ultrastructural analysis of renal tissue, as well as was evaluated the effect on bone marrow. Our result showed an increase in the urea levels (37.5% y 33.5%) with amphotericin B and the analogue, respectively. The synthetic analogue showed morphological alterations in glomerules, renal tubules and mitochondries. As well as were observed the presence of some cells in apoptosis and necrosis. Animals treated with amphotericin B showed great amount of cell in apoptosis as well as membranal and mitochondrial alterations. Any compound induced changes in bone marrow. Our result showed that the synthetic analogue was less toxic than amphotericin B in mice.