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Protective effect of APS on the pathological changes of Muller cells of STZ‐induced T2DM Rats retina
Author(s) -
Li Yuhong,
Ke Min,
Ouyang Jingping
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a434-d
Subject(s) - retina , streptozotocin , diabetic retinopathy , endocrinology , medicine , diabetes mellitus , retinal , retinopathy , pathological , cell , insulin , biology , ophthalmology , neuroscience , biochemistry
To investigate the effects of Astragalus polysaccharide(APS) on the pathological changes of Muller cell of STZ‐induced T2DM rats. The T2DM model was duplicated by the high‐fat diet and small dose streptozotocin(STZ, 25mg/kg,ip).After APS therapy (400mg/kg/d,ig) for 6 weeks, blood sugar, glycosylated hemoglobin and serum insulin were observed. Diabetes‐Induced Dysfunction of Retinal Müller Cells play a very important role in contributing diabetic retinopathy. Muller cells prominently expressed Kir2.1 proteins.we hypothesized that APS would reduce the morbidity of diabetic retinopathy by increasing the Expression of Kir2.1. By using RT‐PCR, we investigated the effect of Protective effect of APS on the pathological changes of Muller cells of STZ‐induced T2DM Rats retina.The expression of Kir2.1 mRNA was downregulated, in the Muller cell of STZ‐induced T2DM rats retina.After APS therapy for 6 weeks, The expression of Kir2.1 mRNA was significant higher than those in the Muller cell of STZ‐induced T2DM rats retina.However these significant alternation did not occur in the two non‐diabetic control groups .APS may have an effect on protecting functions of Muller cell, especially in T2DM rats retina. Through this, APS reduce the morbidity of diabetic retinopathy.