Premium
The effect of cardiac ischemic preconditioning on rat left ventricular gene expression profile
Author(s) -
Canatan Halit,
Benter Ibrahim F.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a419-b
Subject(s) - gene expression , microarray , dna microarray , microarray analysis techniques , ischemic preconditioning , ischemia , medicine , significance analysis of microarrays , gene , andrology , cardiology , chemistry , biochemistry
Ischemic preconditioning (IPC) is a phenomenon where heart is rendered more resistant to subsequent ischemia‐reperfusion (I‐R)‐induced injury by one or more brief episodes of I‐R. The mechanisms responsible for cardio‐protective effects of IPC are not well characterized. The objective of the study was to characterize gene expression profiles in the left ventricles of male Wistar rat hearts exposed to I‐R or IPC followed by I‐R. Three groups were studied (n=8/group): Group 1 was hearts that were perfused for 30 min. Group 2 was perfused hearts that subjected to 40 min I and 30 min R. Group 3 was perfused hearts that were subjected to IPC (5 min I + 10 min R + 5 min I + 10 min R) followed by I‐R. Total RNAs were isolated from left ventricular tissues. Codelink gene expression system (GE Healthcare) was used for cRNA target preparation, hybridization of microarrays (Rat UniSet 10K CodeLink bioarrays) and detection. Microarrays were scanned with Affymetrix 428 Array scanner. Data analyses were carried out with GeneSifter microarray data analysis software. Expression of 140 genes were changed (44 up‐regulated, 96 down‐regulated) due to IR (Group 2) compared to only perfused hearts (Group 1). 23 genes, that were down‐regulated due to I‐R, were up‐regulated by IPC (Group 3). IPC down‐regulated expression of seven genes which were up‐regulated by I‐R. This work was supported by Kuwait University, Research Grant No. RM 01‐04.