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Relationship of the oxidized low‐density lipoprotein (OX‐LDL) receptor, LOX1, to OX‐LDL reactivity of various arterial muscles
Author(s) -
Dominguez Maria H,
Rice Ami E,
Sanders Elmer T,
Temm Connie J,
Packer C Subah
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a410-e
Subject(s) - low density lipoprotein , contraction (grammar) , medicine , scavenger receptor , artery , receptor , endocrinology , lipoprotein , chemistry , cholesterol
Hypertension is associated with metabolic syndrome and diabetes mellitus (NIDDM) although the causal relationship is unknown. Elevated OX‐LDL is characteristic of NIDDM. OX‐LDL causes dose‐dependent arterial muscle contraction but the signaling pathway is not understood. The OX‐LDL receptor (LOX1) has been identified on arterial smooth muscle but its role is unknown. Interestingly, the caudal artery, a muscular systemic vessel, is more reactive to OX‐LDL than is either the conduit aorta or the low pressure pulmonary artery in the rat. The purpose of the current study was to determine if LOX1 density on the three arterial muscles correlates with OX‐LDL reactivity. LOX1 density was evaluated by immunofluorescence and confocal microscopy using a polyclonal antibody raised in rabbits against recombinant LOX1. Quantification was performed using Metamorph software (Universal Imaging). Results show that LOX1 is expressed in sufficient density to be responsible for the initial activation step in the OX‐LDL contraction. In addition, LOX1 density correlates with OX‐LDL reactivity for the three arterial types investigated. Results suggest a role for LOX1 activation in mediating OX‐LDL induced vasoconstriction. (Supported by NIH T35 Grant # HL07802. LOX1 antibody was a gift from JH Dominguez. MH Dominguez was a 2006 NIH T35 Scholar. ET Sanders was a 2006–07 APS Frontiers in Physiology Professional Development Fellow.)