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The expression of FIZZ/resistin/RELM family in mouse hypoxia lung
Author(s) -
Su Qingning,
Johns Brian A,
Johns Roger A
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a405-c
Subject(s) - biology , resistin , lung , hypoxia (environmental) , real time polymerase chain reaction , downregulation and upregulation , gene , microbiology and biotechnology , endocrinology , medicine , genetics , chemistry , insulin resistance , organic chemistry , oxygen , insulin , adipokine
The FIZZ/resistin/RELM family contains 2 human and 4 murine genes. This important gene family has been implicated in a variety of human diseases. In mouse, hypoxia‐induced mitogenic factor (HIMF), also called FIZZ1 or RELMα has been shown to have potent mitogenic, angiogenic and vasoconstrictive effects in the lung vasculature. Among the four members, HIMF and FIZZ4/RELMγ are the most closely related proteins, with 72% identity in amino acid sequence. Whether FIZZ4/RELMγ also plays similar role as HIMF in lung tissue, remains unclear. Using polymerase chain reaction (PCR) and RT (reverse transcriptase)‐PCR, we have analyzed the expression of FIZZ/resistin/RELM family in normal and hypoxic murine lung tissue. Mouse lung cDNA library and lung cDNAs prepared from normoxic and hypoxic mice were used in the PCR. HIMF, FIZZ2/RELMβ and FIZZ4/RELMγ mRNA are shown to be expressed in mouse lung tissue but not resistin. The expression of FIZZ4/RELMγ is the most abundant among the FIZZ/resistin/RELM family in lung tissue and is also upregulated in hypoxic murine lung. Along with HIMF, We have demonstrated that FIZZ4/RELMγ is another member of FIZZ/resistin/RELM family highly expressed in murine lung. Like HIMF, the expression of RELMγ can be regulated by hypoxia. Our results suggest that FIZZ4/RELMγ may be another factor involved in inflammation in lung. ( Funded By: NIH grant HL39706)