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“Probing Single Molecule Dynamics: From In Vitro to In Vivo ”
Author(s) -
Xie Sunney
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a40-a
Subject(s) - in vivo , in vitro , biophysics , molecule , translation (biology) , chemistry , dynamics (music) , transcription (linguistics) , computational biology , microbiology and biotechnology , nanotechnology , biology , biochemistry , messenger rna , physics , materials science , genetics , gene , linguistics , philosophy , organic chemistry , acoustics
Recent advances in single molecular detection and manipulation techniques allow probing dynamics of single enzyme molecules, including binding of substrates, conformational changes, and biochemical reactions. Fundamental biological processes such as transcription, translation, and replication can now be followed in real time on a single molecule basis, not only in vitro, but also in an individual living cell.