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Identification of genes related to obesity in dogs
Author(s) -
Yamka Ryan M.,
Friesen Kim G.,
Gao Xiangming,
AlMurrani Samer
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a4-d
Subject(s) - overweight , lipid metabolism , endocrinology , gene , medicine , biology , fold change , gene expression , metabolism , rna , fatty acid metabolism , obesity , biochemistry
Forty‐two neutered/spayed beagles (average age = 7.5 ± 2.8 years) were identified for this study. Dogs were weighed, given a body condition score (BCS; 1=lean, 3=ideal and 5=obese) and a blood sample was drawn into a PAXgene blood RNA tube. Of the forty‐two beagles identified, 12 were classified as lean (BCS = 2.2 ± 0.3; BW = 10.5 ± 1.9 kg) and 30 as overweight (BCS = 4.3 ± 0.4; BW = 15.2 ± 3.3 kg). RNA was extracted according to the procedures provided in the PAXgene Blood RNA Kit Handbook (Qiagen, Valencia, CA). RNA was hybridized to an Affymetrix Gene Chip Canine‐2 Genome Array and normalized using Robust Multi‐Array Average. Transcripts having a P < 0.01 (following a false discovery rate adjustment value of 0.1) and > 1.3‐fold change were considered different among the two groups. Analysis of the RNA found differences in 815 genes between the two groups. Of the genes identified, 59 were expressed higher and 756 were lower in the overweight group when compared to the lean group. The genes with higher expression in overweight dogs were associated with fatty acid metabolism, purine metabolism and platelet derived growth factor signaling. The genes with lower expression in overweight dogs were associated with nucleotide metabolism, carbohydrate metabolism, interleukin signaling, PPAR signaling, IGF‐1 signaling, insulin receptor signaling, amino acid metabolism, branch chain amino acid degradation and lipid metabolism.