Premium
Identification of genes altered with growth phase nutrition in growing puppies
Author(s) -
Frantz Nolan Z,
Zicker Steve C,
Friesen Kim G,
Yamka Ryan M,
Gao Xiangming,
AlMurrani Samer
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a4-b
Subject(s) - weaning , beagle , gene , biology , immune system , microarray , rna , zoology , physiology , genetics , gene expression
Providing proper nutrition during early growth may influence the propensity to develop disease later in life and is critical for proper development of puppies. Sixteen beagle puppies were allotted to a commercially available food or Hill's Science Diet® from weaning to one year of age. RNA was extracted from blood and hybridized to an Affymetrix GeneChip Canine‐2 Genome Arrays and normalized using Robust Multi‐Array Average. Transcripts having a P < 0.05 (following a false discovery rate adjustment value of 0.1) and a fold change range of at least 1.5 were considered different among the two groups. Analysis of the RNA found differences in 99 genes between the Hill's Science Diet® fed puppies and puppies fed the commercial food. Of the genes identified, 32 were up‐regulated and 67 down‐regulated in Hill's Science Diet® fed puppies when compared to puppies fed the commercial food. Genes associated with, immune system activation, cardiovascular development, lipid metabolism, skeletal and muscular disorders, cellular compromise, and cancer were decreased, while muscle contraction increased in puppies fed Hill's Science Diet® compared to the commercial food.