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Examination of Chromosome 1p Alterations in Glioblastomas
Author(s) -
Kellermier Harry Clayton,
Nikiforova Mari.,
Cieply Kathleen,
Hamilton Ronald L.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a393-a
Subject(s) - loss of heterozygosity , microsatellite , fluorescence in situ hybridization , biology , fish <actinopterygii> , glioblastoma , epidermal growth factor receptor , gene duplication , chromosome , glioma , pathology , cancer research , medicine , gene , genetics , receptor , allele , fishery
Oligodendrogliomas with loss of 1p have an increased response rate to chemotherapy. This has led to increased use of testing for alterations of 1p in diffuse gliomas. At our institution we routinely characterize alterations of 1p by both fluorescent in situ hybridization (FISH) and loss of heterozygosity (LOH) in all gliomas. We noted alterations of 1p in a significant number of glioblastoma multiforme (GBM) cases and analyzed these alterations in a retrospective study of 120 newly diagnosed GBMs. We found 33/120 (27.5%) have LOH on 1p for at least one of 6 microsatellite markers, while only 4/120 (3.3%) showed loss of 1p by FISH. 46/120 (38.3%) of the GBMs showed epidermal growth factor receptor (EGFR) amplification by FISH. LOH of 1p was found in 26/73 (35.6%) of GBMs without EGFR amplification and only 7/47 (14.9%) with EGFR amplification. 13/27 (48.1%) cases with results available for all 6 microsatellite markers show LOH of 1p at D1S407 (1p36.21), which is a microsatellite marker just proximal to the site for the FISH probe. We conclude that alterations of 1p are seen in 27.5% of GBMs, are twice as common in GBMs without EGFR amplification, and involve the distal telomeric microsatellite marker D1S407 (1p36.21) in 48.1% of the cases.