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Mechanism of the ATP‐dependent chromatin remodeling complex ACF
Author(s) -
Narlikar Geeta J,
Yang Janet G,
Racki Lisa,
Partensky Peretz
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a39-a
Subject(s) - nucleosome , chromatin , chromatin remodeling , histone , förster resonance energy transfer , microbiology and biotechnology , mechanism (biology) , biophysics , dna , chromatosome , biology , chemistry , genetics , physics , fluorescence , quantum mechanics
Silent loci are often characterized by regularly spaced nucleosomes. The ATP‐dependent chromatin assembly factor ACF generates these structures, yet how it measures out equal spacing between nucleosomes is not known. We have used fluorescence resonance energy transfer to follow nucleosome movement in real time. We show that ACF senses the length of flanking DNA and transduces this information in an ATP‐dependent manner to regulate the rate of nucleosome movement. Our data also suggests a mechanism to explain previous results indicating a role for the histone H4 tail in the ACF reaction.