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Study the expression of VASP in gastric carcinoma
Author(s) -
Peng Xiaochun,
Zhang Jingwei,
Wei Lei,
Wen Xianmei,
Li Hua
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a386
Subject(s) - motility , western blot , metastasis , pathology , lamellipodium , cell , epithelium , actin , cancer , cell migration , biology , immunohistochemistry , cancer research , medicine , microbiology and biotechnology , biochemistry , genetics , gene
Cell motility has been implicated in the spreading of cancer cells and is an essential step in metastasis. For a single cell, the motility relies on the actin‐based cytoskeleton for both generating protrusions and retracting the rear of the cell in order to generate a motility cycle that results in net translocation. Vasodilator stimulated phosphoprotein (VASP) is required for the assembly of actin filament at cell leading edge to push the plasma membrane and the cell forward antagonizing with Arp2/3 (actin‐related proteins 2 and 3) complex. This study was undertaken to analyse VASP expression in human gastric adenocarcinomas tissues and normal gastric tissue (a standard distance from resected carcinomas) of patients who underwent surgical resection and gave their informed consent. To analyze VASP protein expression, immunohistochemistry and western blot analysises were performed. Carcinomas (epithelium mucosae, glandular epithelium, vascular endothelial cell, vascular smooth muscle cell) have significantly greater VASP expression than normal epithelium. Moreover, VASP expression in adenocarcinomas increasing significantly with more advanced tumor stage. VASP is expressed at higher levels in cancer tissues compared to adjacent tissues in Western blot analysis. The significant increase in the expression of VASP in carcinomas in parallel to pathological staging indicates that it may regulate the invasive behavior of gastric carcinomas as carcinoma invasion is increased in more advanced tumors. (This work was supported by National Natural Sciences Foundation of China under Grant No.30570751)