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Impact Of Medroxyedroxy Progesterone On Expression Of Cell Cycle Regulatory Genes With Metastatic Ability In Breast Cancer
Author(s) -
Taranikanti Varna,
Vuthaluru Seenu,
Roy Tarasankar
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a383-b
Subject(s) - breast cancer , cyclin d1 , cell cycle , progesterone receptor , immunohistochemistry , luteal phase , menstrual cycle , estrogen , hormone , hormonal therapy , cancer research , cancer , medicine , estrogen receptor , gene expression , cyclin e , cyclin , oncology , biology , gene , biochemistry
Breast cancer patients operated during the luteal phase of menstrual cycle tend to have a better survival than those operated in the proliferative phase. Cyclical patterns of immune function, cancer cell division, apoptosis may be affected by the hormonal milieu at the time of surgery. We hypothesized that preoperative hormonal manipulation (hydroxy progesterone) would be able to provide better understanding of the biological mechanisms to account for the patients prognosis. Aim: To study the impact of (hydroxyl progesterone) on the expression of cyclins, P27, Her2neu , ER, PR in early breast cancer patients. Methods:100 breast tumor samples were collected and half were randomized to receive progesterone. Samples were fixed in 10% buffered formalin for immunohistochemistry using monoclonal Ab for ER, PR and Her2. RNA was extracted and subjected to RT‐PCR using primers for cyclin D1, D2 and P27. Results: There was higher expression of cyclin D1 and P27 in both the groups and the expression of cyclin D2 was absent in more than 64 patients. This correlated with the presence of estrogen receptors. However, the expression of Her2 did not show any significant difference in both the groups. Conclusion: Tumor expression of genes may contribute to improved survival with change in the hormonal milieu. However larger studies are required to assess mechanisms of gene regulation and verify any relevant influence in long term survival.