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Diallyl sulfide inhibits WEHI‐3 leukemia cells in Blab/C mice In Vivo
Author(s) -
Yu Fu Shun,
Lin ChinChung,
Yang JaiSing,
Chung JingGung
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a382-c
Subject(s) - in vivo , leukemia , spleen , in vitro , apoptosis , k562 cells , microbiology and biotechnology , chemistry , cancer research , pharmacology , biology , immunology , biochemistry
Diallyl sulfide (DAS), an organosulfur compound from garlic, is recognized as a potential chemo‐preventive agent, and has a wide range of pharmacological effects, including anti‐leukemia cells in vitro. Many investigators have already demonstrated that DAS induces apoptosis from human leukemia HL‐60 and K562 leukemic cells, but there are no reports regarding whether or not it affects leukemia cells in vivo. However, the exact effect of DAS on leukemia cells in murine in vivo is unknown. In this study, we focused on the effects of DAS in vivo affecting murine leukemia WEHI‐3 cells. The primary studies tested the effects of DAS on the changes of percentage of viable cells of WEHI‐3 in vitro by MTT assay and the results showed that DAS decreased the % of viable cells and those effects are dose‐and time‐dependent manners. The following experiments examined the effects of DAS on leukemia WEHI‐3 cells in BLAB/c mice in vivo after i.p. was injected with WEHI‐3 cells before being cotreated with or without DAS, and the results indicate that DAS decreased the percentage of Mac‐3 marker, inhibiting the differentiation of precursor of macrophage and T cells. The final experiments measured the weights of liver and spleen and the results indicated that DAS decreased the weights of liver and spleen. It also brought attention to the enlarged spleens in murine after i.p. was injected with WEHI‐3 cells. However the DAS decreased the size of the spleen. The results showed that DAS affects WEHI‐3 cells in vitro and in vivo. Further investigations are needed to find out the molecular mechanism of DAS on the leukemia mice in vivo.