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Effect Of Human CMV Infection On The Growth And Differentiation Of Embryonic Stem Cell
Author(s) -
Wang Lin,
Ouyang Jingping
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a381-b
Subject(s) - human cytomegalovirus , biology , flow cytometry , embryonic stem cell , cell cycle , gene expression , cell , gene , immediate early gene , apoptosis , virology , real time polymerase chain reaction , cell growth , immunocytochemistry , microbiology and biotechnology , virus , genetics , endocrinology
OBJECTIVE: To establish the cell model of human cytomegalovirus (HCMV) infect embryonic stem cell (ESC) and study the effect of HCMV infection on growth and differentiation of ESC. METHODS: The mouse ESCs (D 3 ) was cultured with HCMV. The expression of HCMV immediate early (IE) gene was detected by immunocytochemistry and RT‐PCR. Gene expression profile of the ESC after infection of HCMV was characterized by using cDNA microarry chip. Apoptosis and cell cycle were determined by flow cytometry. RESULTS: The positive products of HCMV IE protein can be observed in the infected ESC. Expression of HCMV IE gene was detected 24h after infection. The virus‐like granules can be found in the infected ESC under electron microscope. Analysis of gene chip shows that 134 differentially expressed genes were identified. The flow cytometry shows that the percentage of apoptosis does not increase in experiment group than that of control one, but there is more cells accumulation in S phase in experiment group than that in control one. CONCLUSION: Established a cell model of HCMV infect ESC. The developmental disorders caused by HCMV infection may be associated with the changes of gene expression profile and cell cycle.