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Papilla as a Niche for Adult Human Renal Stem Cells
Author(s) -
Ward Heather H,
Roitbak Tamara,
Romero Elsa G,
WandingerNess Angela
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a379-g
Subject(s) - nestin , renal papilla , renal stem cell , stem cell , major duodenal papilla , biology , kidney , pathology , microbiology and biotechnology , dermal papillae , anatomy , progenitor cell , neural stem cell , endocrinology , medicine , hair follicle
With over 58,000 patients in the US on the waiting list for a kidney transplant, the isolation of adult renal stem cells for the treatment of kidney disorders is of significant urgency. Populations of CD133+ primary human kidney cells from the renal cortex and renal papilla were isolated by enzymatic digestion from kidneys unsuited for transplant. CD133+ human papillary cells express the neuronal stem cell marker nestin and may be triggered to adopt tubular epithelial‐ and neuronal‐like phenotypes and exhibit morphologic plasticity through modulation of culture conditions. Papillary cells readily associate with cortical tubular epithelia on co‐culture and in 3‐dimensional collagen gel cultures, papillary cells participate in epithelial morphogenesis with cortical tubular epithelia. In a heterologous mouse metanepthric organ culture system, human papillary cells remain viable and incorporate into developing tubules. In situ, nestin+ and CD133+ cells are intercalated exclusively between loop of Henle tubular epithelia that are located within renal papilla. Thus, as described for rodent kidney (Oliver et al., J Clin. Invest . 2004 114 :–804), the human papilla serves as a niche for cells with the hallmarks of adult human kidney stem cells.