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Ultrasound energy markedly and rapidly effects stem/progenitor cell labeling with nanoparticle beacons for molecular imaging and cell tracking
Author(s) -
Partlow Kathryn C,
Brant Jason A,
Marsh Jon N,
Nolta Jan A,
Hughes Michael S,
Lanza Gregory M,
Wickline Samuel A
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a379-b
Subject(s) - progenitor cell , stem cell , electroporation , flow cytometry , cell , biomedical engineering , cd34 , chemistry , microbiology and biotechnology , medicine , biology , immunology , biochemistry , gene
Labeling stem/progenitor cells for non‐invasive imaging and tracking typically requires long exposure times or adjunctive methods, such as electroporation or transfection agents. Recently, we demonstrated that perfluorocarbon nanoparticles (PFC NP: 200 nm) provide a unique and sensitive label for cell tracking by MRI that entails 12 hours of incubation for efficient cellular labeling. To develop an approach that reduces the prolonged labeling time, stem/progenitor (CD34 + CD133 + CD31 + ) cells derived from human umbilical cord mononuclear cells were incubated with PFC NP and exposed to clinical levels of ultrasound (US) energy with a standard medical imaging system at typical imaging frequencies (2MHz) and power levels (1.9MI). Flow cytometry revealed US application greatly improved labeling in under 1 hour versus cells not exposed to US (55±4% vs. 6±3% respectively, p<0.001), which approaches the labeling efficiency achieved in the 12 hour incubation method (71±4%). No reduction in cell viability due to the labeling procedure was observed when comparing untreated and US‐exposed cells (~90% viable). This unique approach should facilitate investigation of regenerative therapeutics by providing a safe adjunctive method to label cells for real‐time tracking. Furthermore, the labeling procedures can be carried out with conventional ultrasound imaging devices available to most researchers.