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Mesnenchymal stem cells favor healing and remodeling in a canine acute ischemia model
Author(s) -
Vela Deborah,
Silva Guilherme,
Willerson James T.,
Perin Emerson,
Buja L. Maximilian
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a379-a
Subject(s) - medicine , masson's trichrome stain , mesenchymal stem cell , ischemia , pathology , fibrosis , saline , infarction , occlusion , bone marrow , myocardial infarction , cardiology
Objective: To evaluate the effects of allogenic bone marrow mesenchymal stem cells (BM‐MSCs) on healing and remodeling in the subacute phase of a canine acute ischemia model. Methods: Sixteen hearts from a canine 3‐hour coronary occlusion/reperfusion model were excised at 3 weeks, weighed and perfused; infarct size and bed at risk (BAR) were measured. Hearts were sliced into 4 transverse sections from apex to base. Each slice was cut into 9 segments and formalin‐fixed. Paraffin sections from each segment were stained with H&E, trichrome and picrosirius red stains. Seven days post‐occlusion, 10 dogs had received 100 x 10 6 allogenic BM‐MSCs; 6 control dogs received normal saline. All animals were sacrificed at 21 days. Results: Infarct size and BAR was similar in both groups. Fibrosis and unresolved necrotic myocardium in BM‐MSC vs. control was 83.1±10.8 vs. 68.1±13.1 and 17.0±11.1 vs. 31.9±13.1, respectively; p<0.05. Conclusions: Infarcts of treated animals demonstrated more collagen deposition and less unresolved necrotic myocardium compared to controls. Thus, BM‐MSCs delivery in the subacute phase of acute myocardial infarction positively influences the healing and remodeling of the infarct scar.