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Effects of oral antibiotics on lymphocyte response in massive bowel resected rats with small bowel segment reversal
Author(s) -
Lo HuiChen,
Lee ChienHsing
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a376
Subject(s) - medicine , antibiotics , splenocyte , lymphocyte , oral administration , gastroenterology , spleen , biology , microbiology and biotechnology
Using a massive bowel‐resected rat model, our previous study demonstrated that oral antibiotics significantly attenuated the surgical reversal‐induced increases in white blood cell count and serum concentrations of nitric oxide and interleukin‐6 without affecting the intestinal histology. In the present study, we further investigated the effects of oral antibiotics on the responses of peripheral blood leukocytes, splenocytes and thymocytes. Male Wistar rats were performed with a 70% small bowel resection with or without a 3‐cm small bowel segment reversal or performed with a sham‐operation. After surgery, half numbers of animals with surgical reversal were orally administered with clindamycin (50mg/kg/day) plus amoxicillin (50mg/kg/day) for 3 weeks. Our results showed that oral antibiotic administration did not alter the surgical reversal‐induced decreases in the percentages of B splenocytes and T helper thymocytes. In contrast, oral antibiotic administration significantly improved surgical reversal‐induced alterations in the ability of cell proliferation in thymocytes and the production of TNF‐α in peripheral blood B cells, as well as significantly increased the percentage of granulocytes in the splenocytes. These results suggest that oral antibiotics may be used to attenuate the inflammatory response and to improve, at least partially, the lymphocyte functions in massive bowel resected rats with small bowel segment reversal.