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A rat model for studying the postprandial appearance of vascular endothelial dysfunction
Author(s) -
Magne Joëlle,
Mariotti François,
Tome Daniel,
Huneau JeanFrançois
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a375-b
Subject(s) - postprandial , medicine , endocrinology , endothelial dysfunction , hemodynamics , meal , endothelium , diabetes mellitus
In Humans, high‐fat or high sucrose meals induce transient vascular endothelial dysfunction, which is the hallmark of early atherogenesis. However, little is still known regarding how postprandial metabolic challenge by nutrients translates into vascular risk. An animal model to monitor the postprandial vascular function under different conditions would be useful. In ten conscious Wistar Kyoto rats, we have developed a hemodynamic test of endothelial function, based on the rapid, transient, dose‐dependent blood pressure decrease after iv acetylcholine, which was blunted by prior administration of L‐NAME. This test proved to detect the impairment of vascular reactivity 6h after a methionine load (350mg/kg). Then, we measured vascular reactivity before and 2, 4 and 6 hours after single high fat meal. Vascular reactivity fell significantly in the postprandial state (p<0.05) being 29 ± 16% and 54 ± 10% lower than baseline at 4h and 6h after meal, respectively. Plasma triglycerides strongly correlated with vascular reactivity (R²=0.9852, p<0.01). The high fat load significantly increased plasma IL‐6 and PAI‐1 (from 2 hours after the meal) but did not affect plasma sICAM‐1. This rat model should be useful to study the effect of nutrients and diets on vascular risk by analysing the postprandial metabolic and physiological adverse effects.