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Effect of oligosaccharide from brown algae on apoptotic activity in mouse thymocytes
Author(s) -
Ryu Duck Sun,
Kim KiHoon,
Choi Hyunju,
Lee Dong Seok
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a373-a
Subject(s) - apoptosis , laminarin , biology , microbiology and biotechnology , flow cytometry , population , immune system , annexin , brown algae , biochemistry , immunology , algae , botany , enzyme , demography , sociology
This study was performed to examine anti‐apoptotic activity of oligosaccharide prepared from brown algae in mouse thymocytes. Laminarin oligosaccharide from brown algae (LOBA) was derived with an endo‐(‐(1(3)‐glucanase from Bacillus circulans. Mouse thymocytes were treated with LOBA (1, 2, 3, 4 mg/ml) and assayed quantitatively by Annexin V technique using flow cytometry. LOBA significantly suppressed apoptotic death and extended the cell lifespan in dose‐dependent manner. The early apoptosis population decreased more in LOBA treated cells compared with control cells. And the late apoptosis and necrosis population also decreased in those LOBA treated cells. Gene expression responding to LOBA was examined in the cultured mouse thymus cells by cDNA microarray. It was found that the genes coding for the immune response proteins, IL6, IL7, and IL15 were induced significantly. In conclusion, laminarin oligosaccharide from brown algae has anti‐apoptotic activity with an apparent relationship to the genes of immune response proteins. (This work was supported by KRF 2005‐F00014, BK21, NURI, and Inje‐FIRST)