Premium
Intestinal/hepatic mRNA Fluctuations of Polyamine Related Genes During Nutritional Rehabilitation of Undernourished Rats with Casein and Modified Soy Protein
Author(s) -
Abraham Wall,
Barca Ana M Calderón,
Gloria YépizPlascencia
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a372
Subject(s) - messenger rna , proliferating cell nuclear antigen , polyamine , ileum , casein , biology , medicine , endocrinology , cell growth , gene , microbiology and biotechnology , biochemistry
Polyamines are involved in cell proliferation and are tightly controlled by p53, PPARa and diet. Steady state mRNA levels in liver, jejune and ileum of ODC, AdometDC, SpdS, SpmS, SSAT, PPARá, OAZ and OAZi and histological changes in intestine during nutritional rehabilitation of undernourished rats with enzymatically modified soy protein (MSP18%) or casein (CAS18%) based diets, were evaluated. Transcript levels were evaluated by RT‐PCR using GAPDH as constitutive gene, and proliferating cell nuclear antigen (PCNA) and p53 immunochemically detected at 0, 1, 2 and 3rd week of nutritional rehabilitation. Before recovery, undernourished rats had lower mRNA levels than control rats of almost all genes, except for SSAT, OAZ and PPARá in liver and OAZ and the last two in ileum. Until 2nd week of recovery, MSP18% fed rats showed higher AdometDC mRNA level than CAS18% group in intestinal segments. PPARá mRNA levels behave differently in each tissue but there was no association with corresponding mRNA levels for SpdS and SSAT. Small differences in mRNA levels for all other genes were diet specific but normalized until 3rd week. There were no diet related differences in proliferating markers during recovery. Despite their equal efficiency to enhance cell growth rate and organ functionality, CAS and MSP excerpt distinct effects on transcriptional activation of polyamine metabolism related genes αα