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Effects of tea catechins on lipolysis and UCP1 mRNA expression in rat adipose tissues
Author(s) -
Nomura Sachiko,
Ichinose Takashi,
Jinde Manabu,
Kawashima Yu,
Shimada Chiemi,
Tachiyashiki Kaoru,
Imaizumi Kazuhiko
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a368-c
Subject(s) - lipolysis , medicine , endocrinology , thermogenesis , brown adipose tissue , white adipose tissue , thermogenin , adipose tissue , adipocyte , chemistry , messenger rna , biology , biochemistry , gene
The present study was designed to investigate the effects of dietary tea catechin (TC) intake on lipolysis of white adipose tissue (WAT) and on the mRNA expression of uncoupling protein 1 (UCP1), which is a key protein of thermogenesis in brown adipose tissue (BAT) of rats. Five‐week‐old male Sprague Dawley rats were fed with 35% (w/w) high fat diet (HF) for 5 weeks. Thereafter, the animals were divided into 4 groups ; HF, HFTC (HF + 0.5% TC), NF (5% normal fat diet) and NFTC (NF + 0.5% TC). The animals were fed with each diet for 8 weeks. Epididymal WAT and interscapular BAT were collected on the final day of the experiment. Adipocytes were isolated from WAT to assess the lipolytic responses to β‐adrenoceptor agonist, isoproterenol (ISO), and cAMP analogue, dibutyryl cAMP (DiBcA). UCP1 mRNA levels in BAT were quantified by real‐time RT‐PCR method. Both of ISO (1mM) and DiBcA (1μM) heightened lipolysis of WAT adipocytes in all of the 4 groups. However, no significant differences were observed in the degrees of lipolysis between NF and NFTC. Similar results were shown in the lipolysis between HF and HFTC. In contrast, dietary TC intake increased significantly UCP1 mRNA expression in BAT of NFTC group as compared to that of NF group while the difference between HF and HFTC groups was not significant. These results suggest that BAT rather than WAT plays an important role as the target for the suppressive effect of TC on body fat accumulation.