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Resveratrol Inhibits Adipogenesis and Induces Apoptosis in 3T3‐L1 Adipocytes
Author(s) -
Rayalam Srujana,
Yang JeongYeh,
DellaFera Mary Anne,
Park Hea Jin,
Ambati Suresh,
Baile Clifton A
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a365-c
Subject(s) - adipogenesis , resveratrol , apoptosis , oil red o , viability assay , adipose tissue , adipocyte , endocrinology , medicine , chemistry , 3t3 l1 , biology , biochemistry
Adipose tissue mass can be decreased by inhibiting adipogenesis and by removal of both preadipocytes and mature adipocytes by apoptosis. We investigated the effects of resveratrol, a flavonoid, on adipogenesis and apoptosis using 3T3‐L1 cells. Preadipocytes and mature adipocytes were treated with resveratrol (R) at 50 and 100 (M. Viability was measured by MTS assay; apoptosis was measured by single strand DNA ELISA. In preadipocytes, R50 and R100 decreased cell viability by 15.2 ± 3.6 and 24 ± 1.7 % (p<0.001) of control respectively after 48 hours. In mature adipocytes R50 and R100 decreased cell viability by 11.9 ± 2.1 and 21 ± 3 % (p<0.001) of control respectively after 48 hours. However, R50 did not induce apoptosis in either preadipocytes or mature adipocytes, whereas R100 increased apoptosis in mature adipocytes by 46 ± 8.7% (p<0.001) more than control after 48 hours. The effect on apoptosis in mature adipocytes was also confirmed with Hoechst staining. Resveratrol's effect on adipogenesis was examined by AdipoRedTM assay and Oil red O staining after treatment during differentiation. Resveratrol at 25 and 50 (M concentration significantly decreased lipid accumulation by 44±1.2% and 96.3±0.3% of control respectively (p<0.001). These results show that resveratrol acts directly on adipocytes to reduce cell viability and adipogenesis and induce apoptosis and thus may have applications in the treatment of obesity.