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Anti‐inflammatory effect of caffeic acid, rutin and hypoxoside in C3H mouse model of colitis
Author(s) -
Ye Zhong,
Henderson Abigail,
Liu Zhiping,
Lee Kwangwon,
Lee SunOk,
Wannemuehler Michael J.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a361-a
Subject(s) - caffeic acid , rutin , colitis , inflammatory bowel disease , medicine , myeloperoxidase , ulcerative colitis , caffeic acid phenethyl ester , gastroenterology , inflammation , pharmacology , chemistry , antioxidant , biochemistry , disease
Susceptibility to inflammatory bowel diseases (e.g. Crohn's disease, ulcerative colitis) involves interactions of the genetic predisposition of the individual and mucosal inflammation. We hypothesized that bioavailable Echinacea and Hypericum phenolics, caffeic acid and rutin, as predicted by anaerobic cecal degradation screening, and hypoxoside (an anti‐inflammatory phenolic from African potato) suppressed intestinal damage in a C3H mouse model of colitis. Colitis was induced in C3H/HeOuJ mice (8 wk old, 6 male/6 female per treatment) with 1.25% dextran sodium sulfate (DSS) in drinking water. Rutin or caffeic acid (300 μmol/kg diet) or hypoxoside extract (15 mg/d), were given for 7 d before and during DSS (6 d), as well as without DSS treatment as controls. Colon length, mucosal myeloperoxidase and histopathology scores were compared statistically across treatments to determine the efficacy of the herbal components. Rutin and caffeic acid prevented body weight loss. The colon lengths in phenolic‐treated mice (rutin, caffeic acid and hypoxoside) were longer than in DSS‐treated controls. Myeloperoxidase was inhibited with each phenolic treatment compared with DSS treated controls. Histological scores of the DSS‐treated mice were significantly more severe compared with negative controls. Only caffeic acid caused significantly less severe microscopic lesion scores in colon and cecum than DSS‐treated controls based on mucosal height, necrosis, edema, erosion and inflammatory cell infiltration. Thus, caffeic acid‐containing foods and herbs might protect against colitis. Funded by NIEHS Grant No. PO1 ES12020, Center for Research on Dietary Botanical Supplements, Iowa State University.