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Efficacy of Dietary Polyphenols in the Suppression of Ovalbumin‐Induced Allergic Responses in Female Balb/c Mouse
Author(s) -
Lee EunJu,
Ji Geun Eok,
Sung MiKyung
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a360-e
Subject(s) - ovalbumin , immunoglobulin e , allergic response , sensitization , quercetin , proinflammatory cytokine , chemistry , immunology , kaempferol , balb/c , antibody , pharmacology , inflammation , medicine , antigen , biochemistry , immune system , antioxidant
The allergic reaction is often marked by the production of immunoglobulins including IgE, which is responsible for sensing the allergen and triggering allergic reactions to produce proinflammatory cytokines. The purpose of this study was to investigate anti‐allergic effects of major flavonoids, a group of potential anti‐inflammatory agents. Ovalbumin (OVA,10mg) with 10ug cholera toxin were gavaged to female Balb/c mice. Animals were fed diet containing 0.1 to 0.5% flavonoids for 8 wks starting 1wk before the initial sensitization. Serum level of Igs and Th1/Th2 type cytokines were determined. Serum β‐lactoglobulin (βLG) concentration as a marker of gut permeability was measured. Animals sensitized with OVA showed increased serum levels of total IgG 1 , IgE, OVA‐specific IgG 1 , and OVA‐specific IgE. Quercetin intake decreased the levels of all Igs except IgG 2a and OVA‐specific IgG 2a . Most of flavonoids used inhibited the TNF‐α release, whereas IL‐ 4 was decreased only in quercetin‐fed mice. The amount βLG was significantly higher in sensitized animals and was decreased in 0.1% kaempferol‐fed mice. In conclusion, quercetin may inhibit the allergic responses through the inhibition of TNF‐α and IL‐4 release. Kaempferol possibly decrease intestinal allergen permeability thereby to suppress allergic responses. This study was supported by a grant of the Korea Ministry of Health and Welfare (0405‐FS00‐0501‐001).