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Induction of cell cycle arrest in DU145 human prostate cancer cells by the dietary compound isoliquiritigenin (ISL)
Author(s) -
Lee Yeomyeong,
Choi Hyun Ju,
Jung Jae In,
Park Jung HY
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a360
Subject(s) - du145 , cyclin dependent kinase , cyclin dependent kinase 1 , cell cycle , cyclin b1 , cyclin a , cyclin b , cyclin dependent kinase 2 , cdk inhibitor , cyclin e , cyclin , cell growth , cancer research , chemistry , biology , microbiology and biotechnology , cell , cancer cell , cancer , medicine , biochemistry , lncap
ISL, 4,2′,4′‐trihydroxychalcone, is present in licorice, shallot and bean sprouts and is known to have anti‐tumorigenic activities. In the present study, we examined ISL‐mediated regulation of cell cycle progression in DU145 human prostate cell line. The percentage of cells in G1 phase was increased in cells treated with ISL for 2 h. ISL decreased the protein levels of cyclin D1, cyclin E and cyclin‐dependent kinase (CDK)4. Cyclin A and CDK2 expressions were not altered in cell treated with ISL. The expression of the CDK inhibitor p27 KIP1 was increased in cells treated with 20 μmol/L ISL. In addition, treatment of cells with 20 μmol/L ISL for 24 h led to G2/M cell cycle arrest. Cell division control (CDC)2 protein levels remained unchanged. The protein levels of phospho‐CDC2 (Tyr15) and cyclin B1 were increased and Cdc25C was decreased by ISL in dose‐dependent manners. We demonstrate that ISL promotes cell cycle arrest in DU145 cells providing insight about the mechanisms underlying its antitumorigenic activities.