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Ellagic acid decreases proliferation and IGFBP‐2 in LNCaP human prostate cancer cells
Author(s) -
Okeke Joy C,
Zuniga Krystle,
Meserve Lee,
Hentges Dawn,
Smas Cynthia,
Houston M Sue
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a359-b
Subject(s) - lncap , prostate cancer , cell growth , ellagic acid , cancer cell , chemistry , viability assay , endocrinology , growth factor , cancer , medicine , cell , growth inhibition , cancer research , biology , biochemistry , antioxidant , receptor , polyphenol
Ellagic acid (EA), a phenolic compound found in berries and nuts, elicits important cellular activities in a variety of cancer cells. The present study determined the effects of EA on proliferation of human androgen‐dependent prostate cancer (LNCaP) cells. Additionally, the effect of EA on insulin‐like growth factor binding protein‐2 (IGFBP‐2), a growth factor implicated in prostate cancer, was determined by an enzyme‐linked immunosorbant assay (R&D Systems, Minneapolis, MN). EA significantly inhibited cell proliferation of LNCaP cells in a dose‐dependent manner. Cell viability (MTS, Promega, Madison, WI) was significantly less for EA > 0.1 μM compared to vehicle (0.016% DMSO, p<0.05). Cell counts were 89, 72, 68, 38, and 29% of control for 0.1, 1, 2, 10, and 100 μM EA, respectively. IGFBP‐2 secretion into culture medium decreased with increasing dose of EA (8.7, 10.3, 8.9, 7.3, 5.1 ng/mL/500,000 cells, p< 0.05) for vehicle, 0.1, 1, 2, and 10 μM EA exposure for 3 d, respectively. The present study demonstrated dose‐dependent effects of EA in decreasing cell proliferation and suppressing IGFBP‐2 in LNCaP cells. Doses of EA used in the current study were less than those used in previous reports, and approximate EA concentrations reported in human serum. The potential benefits of modifying cancer cell growth and IGFBP‐2, via dietary bioactive compounds such as EA warrant further investigation.