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Long‐term effects of dietary long chain polyunsaturated fatty acids on bone in healthy rats
Author(s) -
Mollard Rebecca Christine,
Weiler Hope Alberta
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a357-a
Subject(s) - docosahexaenoic acid , polyunsaturated fatty acid , eicosapentaenoic acid , arachidonic acid , femur , medicine , bone mineral , endocrinology , osteoporosis , fatty acid , zoology , chemistry , biology , surgery , biochemistry , enzyme
Long‐term effects of long chain polyunsaturated fatty acids (LCPUFA), arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on bone mass are unclear. Objective: determine how AA and EPA would affect bone when introduced at different points or maintained throughout life. Male and female rats (n=108) received control or one of two LCPUFA diets: AA diet (0.5 g AA, 0.1 g EPA and 0.1 g DHA /100 g of fat) or EPA diet (0.5 g EPA, 0.1 g AA and 0.1 g DHA /100 g of fat). LCPUFA diets were fed in 4 patterns: early (4–18.9 wks old), mid (19–34.9 wks old) or late (35–49 wks old) introduction or continuously (4–49 wks old). Serial measurements included: bone area (BA), bone mineral content (BMC) and urinary Ca. End point measurements included: femur morphometry and mineral composition. In a diet by sex effect on femur BA (P=0.0214), neck width (P=0.024) and head width (P=0.024), males fed EPA had lower BA and males fed AA either mid or continuously had larger femur neck and head widths compared to control. In a diet by gender interaction females fed the EPA diet had lower urinary Ca (P=0.0030) compared to those fed the AA diet. Dietary LCPUFA had varied affects on femur Mg (P=0.0362), Zn (P=0.0107) and P (P=0.0202). Whole body and lumbar spine BMC and BA and femur BMC were not affected by diet. Whether changes in femur size, morphology and mineral composition influence bone strength and risk of osteoporosis requires further study. Funded by ILSI, NSERC and MICH.