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Bixin uptake by blood lipoproteins and leukocytes in dogs
Author(s) -
Chew Boon P,
Hayek Michael G.,
Ceddia Michael A.,
Reinhart Gregory A,
Park Jean Soon
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a352-d
Subject(s) - chemistry , beagle , pharmacology , chromatography , medicine , biology
Bixin may possess anti‐inflammatory action. We studied bixin uptake in dogs given a single dose or repeated doses. In Exp. 1, Beagle dogs (2 yr old; n = 6/dose) were dosed with 0, 5, 10, 20 or 40 mg bixin. Blood was taken at 0, 0.5, 1, 2, 4, 8 and 16 h post‐administration. Because norbixin uptake was low, only data on bixin are reported. Plasma bixin increased dose‐dependently, with peak concentrations of 7, 11, 53 and 79 nmol/L in dogs fed 5, 10, 20 and 40 mg bixin, respectively. Peak bixin concentration was observed at 0.5 h after dosing, with an elimination half‐life of 3.5 h. In Exp. 2, dogs were given the same bixin doses daily for 14 d. Blood was taken 1 h after dosing on d 0, 1, 2, 4, 6, 10 and 14 to study bixin concentrations in plasma. Maximal bixin concentrations was observed on d 1 in dogs fed 20 and 40 mg bixin, reaching steady‐state concentrations of 30–60 nmol/L. Plasma bixin concentrations in dogs given 10 mg bixin continued to increase with time and reached a maximum concentration of 54 nmol/L on d 14. Bixin was mainly distributed in the HDL and LDL fractions, and was approximately 2 to 3 times higher in the HDL, LDL and VLDL fractions on d 14 than on d 6. Bixin was taken up in a dose‐ and time‐dependent manner by the nucleus, microsomes and mitochondria; the mitochondria accounted for 67–85% of total bixin uptake by blood leukocytes, with about equal distribution between the nuclei and microsomes.