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Production of 14 C tomato carotenoids from ‘VFNT’ Cherry cell suspension cultures treated with herbicidal enzyme inhibitors
Author(s) -
Engelmann Nancy J.,
Rogers Randy B.,
Lila Mary Ann,
Erdman John W.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a352-c
Subject(s) - phytoene , carotenoid , lycopene , phytoene desaturase , phytoene synthase , biochemistry , lycopersicon , bioavailability , chemistry , carotene , food science , sucrose , enzyme , biosynthesis , biology , pharmacology , botany
14 C‐radiolabeled carotenoids are useful tools in determining the biodistribution, bioavailability, and metabolism of dietary carotenoids; however radiolabeled tomato carotenoids are generally not commercially available or affordable. Tomato ( Lycopersicon esculentum ‘VFNT’ Cherry) suspension cultures have previously been shown to produce phytoene (PE) and phytofluene (PF) with modest 14 C incorporation when provided with 14 C‐sucrose for all 14 d of the growth period and treated with the herbicidal phytoene desaturase inhibitor norflurazon (NORF) at day 7. In order to optimize 14 C labeling efficiency, cell cultures were clonally selected for increased mass accumulation, 14 C‐sucrose dose duration was varied from 14 d to 7 d, and the lycopene cyclase inhibitor/phytoene synthase upregulator 2‐(4‐chlorophenyl‐thio) triethylamine (CPTA) was given in combination with NORF. Cultures provided with 14 C‐sucrose and treated with CPTA and NORF accumulated radiolabeled PE, PF, lycopene, gamma‐carotene, and zeta‐carotene while NORF alone treated cultures accumulated only PE and PF. Labeling efficiency was evaluated by assessing the radioactivity detected in the culture media, the tomato cells, in specific carotenoids, and that captured from respiration. (Supported by NIH/NCI CA 112649‐01A1)