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Folate intake and the MTHFR C677T genotype influence choline status in young Mexican American women
Author(s) -
Abratte Christian,
Wang Wei,
Li Rui,
Moriarty David,
Caudill Marie
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a347-b
Subject(s) - methylenetetrahydrofolate reductase , choline , phosphatidylcholine , genotype , medicine , homocysteine , sphingomyelin , endocrinology , betaine , chemistry , biology , phospholipid , biochemistry , cholesterol , gene , membrane
Numerous studies have reported a relationship between folate status, the methylenetetrahydrofolate reductase (MTHFR) 677 C>T variant, and disease risk. Although folate and choline metabolism are inter‐related, only limited data are available on the relationship between choline and folate status in humans. This study sought to examine the influences of folate intake and the MTHFR 677 C>T variant on choline status. Mexican American women (n=43; 14 CC, 12 CT and 17 TT) consumed 135 mcg/d as dietary folate equivalents (DFE) for 7 weeks followed by randomization to 400 or 800 mcg DFE/d for 7 weeks. Throughout the study, total choline intake remained unchanged at ~ 350 mg/d. Plasma concentrations of betaine, choline, glycerophosphocholine, phosphatidylcholine and sphingomyelin were measured via LC‐MS/MS for weeks 0, 7 and 14. Phosphatidylcholine and sphingomyelin declined (P=0.001, P=0.009 respectively) in response to folate restriction and increased (P=0.08, P=0.029 respectively) in response to folate treatment. The increase in phosphatidylcholine occurred primarily in response to 800 mcg DFE/d (week x folate interaction, P=0.017). The response of phosphatidylcholine to folate intake appeared to be influenced by MTHFR C677T genotype. The decline in phosphatidylcholine during folate restriction occurred primarily in women with the CC or CT genotype and not in the TT genotype (week x genotype interaction, P=0.089). Moreover, when examined independent of folate status, phosphatidylcholine was higher (P<0.05) in the TT genotype relative to the CT genotype. These data suggest that folate intake and the MTHFR C677T genotype influence choline status in humans. Supported by the NIH grant S06GM53933 and funds from the California Agricultural Research Initiative.