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Lack of interactive effects of exogenous cholesterol and fat on SREBP in pigs of divergent background during postnatal development
Author(s) -
McWhinney Velva Jean,
Godwin Jennifer J,
McWhinney Dalton Rudolph
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a340-c
Subject(s) - sterol regulatory element binding protein , cholesterol , endocrinology , medicine , sterol , biology , zoology , chemistry
Cholesterol rate limiting enzyme, 3‐hydroxy‐3‐methylglutaryl‐CoA reductase, is only partially reduced in pigs fed an exogenous source of cholesterol prior to 25 days postnatal. The study was conducted to examine the relationship between an exogenous source of cholesterol and fat on Sterol Regulatory Element Binding Proteins (SREBP) 1 and 2 in obese and lean pigs. Western blot analyses were preformed to compare the level of precursor and nuclear SREBP 1 and 2 between three day old suckling pigs removed from the sow and place on high fat corn oil or coconut oil diet containing cholesterol and no cholesterol and pigs allowed to suckle for 25 days postnatal. The abundance of nuclear SREBP 1 and 2 was greater than the precursor levels of SREBPs in all pigs. With the exception of lean pigs that suckled for 25 days, precursor SREBP 1 was the same in the pigs regardless to type of exogenous fat added with or without cholesterol and sows milk. Nuclear SREBP 1 and 2 were higher in suckled lean pigs than pigs fed the other dietary regimen and suckled obese pigs. This study suggests that during postnatal development exogenous fat and cholesterol has little impact on nuclear and precursor SREBP 1 and 2 in pigs of divergent background. The increase in nuclear SREBP 1 and 2 in suckled lean pigs suggest a response to a component in sow's milk that promotes cleavage of precursor SREBPs to mature SREBP.