Premium
A high fat, high protein diet increased stearoyl‐CoA desaturase expression in milk replacer fed calves
Author(s) -
Campbell Davina E.,
Lengi Andrea J.,
Akers R. Michael,
Daniels Kristy M.,
Knowlton Katharine F.,
Hill Stephanie R.,
Pearson Ronald E.,
Corl Benjamin A.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a340-a
Subject(s) - adipose tissue , stearoyl coa desaturase , medicine , small intestine , endocrinology , subcutaneous fat , zoology , chemistry , food science , biology , biochemistry , gene expression , gene
Stearoyl‐CoA desaturase (SCD) is an endoplasmic reticulum enzyme responsible for production of monounsaturated fatty acids from saturated fatty acids and mainly utilizes palmitoyl‐ and stearoyl‐CoA as substrates. The purpose of this study was to investigate the tissue distribution of SCD in calves and determine the effects of dietary fat. Calves (n=6/trt) were fed 1 of 4 milk replacers for 9 wks: daily intake of 0.4 kg/d 20% protein, 20% fat (20:20), 0.97 kg/d 28:20 , 0.97 kg/d 28:28L , or 1.46 kg/d 28:28H . Average daily gains were 0.38, 0.61, 0.59, and 0.76 kg/d for 20:20, 28:20, 28:28L, and 28:28H, respectively. Stearoyl‐CoA desaturase protein was detectable in subcutaneous adipose tissue (AT), perirenal AT, and omental AT but was not detectable in liver or small intestine samples in calves fed the 28:28H diet. Stearoyl‐CoA desaturase protein abundance in subcutaneous AT was 4‐fold greater in calves fed 28:28H diet compared to 20:20 diet and 2‐fold greater compared to the 28:20 and 28:28L diets. In conclusion, SCD was not detected in liver or small intestine samples and AT SCD expression was increased in 28:28H diet. Supported by Land O'Lakes and the Virginia Agricultural Experiment Station.