Premium
Weekly Changes in Finger‐Tip Prick Blood Highly Unsaturated Fatty Acid (HUFA) Composition with Acute Fish oil Supplementation and Washout in Men and Women
Author(s) -
Metherel Adam H.,
Armstrong Jeff M.,
Stark Ken D.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a338-d
Subject(s) - eicosapentaenoic acid , docosahexaenoic acid , fish oil , medicine , zoology , washout , venous blood , fatty acid , whole blood , polyunsaturated fatty acid , fish <actinopterygii> , biology , biochemistry , fishery
Finger‐tip prick blood (FTPB) collection is a potential research tool for monitoring eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The fatty acid composition of weekly FTPB samples collected from males and females in response to 4 week fish oil supplement intervention (providing 3.2 g EPA and 1.6 g DHA daily) followed by an 8 week washout period were examined. The fatty acid composition of plasma, red blood cell (RBC), and whole blood (WB) specimens collected by venous puncture at weeks 0, 4, 8 and 12 were analyzed for comparison. Nine males (22.4 ± 1.2 yrs, mean ± SD) and seven females (22.1 ± 1.8 yrs) completed the study. The percentage of n‐3 HUFA in total HUFA (n‐3 HUFA score) at baseline were similar for all blood samples (approximately 21–24). The n‐3 HUFA score in FTPB samples increased from week 0 (21.5 ± 2.2) to week 4 (51.8 ± 3.7) and decreased by week 12 (27.0 ± 1.5) but remained higher than week 0 (p < 0.05). FTPB and WB n‐3 HUFA scores were similar across time. In plasma, the n‐3 HUFA score increased 2.8 fold from week 0 (20.8 ± 2.2) to week 4 (57.7 ± 6.4) but rapidly returned to baseline at week 12 (24.3 ± 2.8). The RBC n‐3 HUFA score increased 1.6 fold from week 0 (23.6 ± 2.3) to week 4 (37.7 ± 3.0), but remained relatively high at week 12 (31.7 ± 2.4). In conclusion, finger‐tip prick blood resembled venous puncture whole blood supporting further development of FTPB collection as a fatty acid research tool.