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Proline, in a commercial total parenteral nutrition (TPN) solution, is inadequate in meeting the metabolic requirements of the parenterally‐fed neonatal piglet
Author(s) -
Pendlebury Christine D,
Urschel Kristine L,
Pencharz Paul B,
Ball Ronald O
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a336
Subject(s) - proline , parenteral nutrition , amino acid , phenylalanine , chemistry , arginine , medicine , zoology , biochemistry , biology
Mammalian milk contains large amounts of proline (73 to 106 mg/g total amino acid). Some TPN solutions contain less than milk would provide. These solutions do not appear to have been specifically tested for proline adequacy in vivo. Recent studies, using the neonatal piglet as a model for the human infant, found that a TPN solution modelled on a commercial product may not contain sufficient proline (2.8g/100g, 0.43g/kg*d), despite apparently adequate arginine. Male neonatal piglets (n=5, ~1.8kg) were implanted with a jugular catheter for diet and isotope infusion and femoral catheter for blood sampling. They received a TPN solution with an amino acid pattern similar to a commercial (control) product and the same solution supplemented with proline (PRO+; 8.2g/100g, 1.25g/kg*d). On d5, piglets received either the control or PRO+ diets for 24h, followed by a primed, constant infusion of 14 C phenylalanine (PHE) for 4h. On d6 piglets received the other diet and underwent another infusion on d7. Breath and blood samples were taken to measure PHE flux, oxidation and plasma amino acid concentrations. Plasma proline concentrations (μmol/L) increased significantly (P<0.01) when the diet was supplemented with proline, bringing the plasma concentration within the sow‐fed reference range. PRO+ reduced the percent dose of PHE oxidized in all piglets yet no significance was found. These results suggest that the proline concentration of this TPN solution was inadequate in meeting the metabolic requirements of the neonatal piglet.