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Conjugated linoleic acid effects on specific adipose depots and muscles and the calpain system in geriatric male mice
Author(s) -
Carroll Shan H,
Sanders Sara R,
Rhoads Robert P,
Thompson Valery F,
Goll Darrel E,
Baumgard Lance H
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a335-b
Subject(s) - conjugated linoleic acid , adipose tissue , endocrinology , medicine , chemistry , calpain , linoleic acid , biochemistry , biology , fatty acid , enzyme
Conjugated linoleic acid (CLA) slightly increases carcass protein in aged mice (Baker et al., 2005), but the biological mechanism by which this occurs is unknown. Geriatric (23 mo) male C57BL/6 mice were fed isoenergetic diets containing 0 or 1.68% methyl esters of CLA (50/50 cis ‐9, trans ‐11 and trans ‐10, cis ‐12) and 6 mice/treatment were harvested at 0, 1, 2, 4, 20 and 30 d post treatment feeding. CLA reduced (18%) feed intake for the first 7 d but did not alter intake thereafter. CLA decreased (12%) BW at d 4 but BW did not differ at other time points. Neither time nor CLA affected the wet weight (%BW) of the pectoral, gastrocnemius and soleus muscles nor the gastrocnemius muscle protein content. CLA had no effect on the gastrocnemius muscle m‐calpain content or on total calpain activity. CLA decreased (~23%) the wet weight (%BW) of subcutaneous and perirenal fat by d 2 and these depots were reduced (>70%) by d 30. CLA reduced (76%) the epidydimal fat pad weight starting at d 20 and it was nearly immeasurable by d 30. There was no dietary effects on heart, spleen or kidney wet mass but CLA increased liver wet weight (%BW) starting on d 20 (87%). CLA had no effect on muscle weights or components of the calpain system, but markedly and quickly reduced the mass of adipose depots in these aged male mice. Further investigation is required to better understand the apparent inconsistencies with regards to CLA effects on protein and muscle metabolism.