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The effect of leptin on metabolic rates is enhanced in mice fed a high calcium diet.
Author(s) -
Mistry Anahita M
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a320-c
Subject(s) - leptin , endocrinology , calcium , medicine , chemistry , metabolic rate , body weight , biology , obesity
Our earlier studies have demonstrated that a single intracerebroventricular (ICV) injection of leptin (1 ug) rapidly stimulates metabolic rates and reduces food intake 3 h after injection. Composition of diet plays a vital role in regulating body weight. Emerging data indicates an inverse relationship between calcium consumption and body weight. The impact of dietary components on the effectiveness of leptin to increase metabolic rates and thus lower body weight is not known. This study was therefore designed to test the efficacy of leptin to alter metabolic rates in mice that were fed diets varying in calcium. C57BL6J male mice were fed modified AIN 93 M diets with 0.25, 0.5, 1.5 or 2.5 % of calcium for 3 wks. After this period, mice were food deprived for 4 h and injected ICV with artificial cerebrospinal fluid (ACSF) or leptin (1 ug). Oxygen consumption of mice, as a reflection of metabolic rate was measured 3 h later. Compared with mice injected with ACSF leptin elevated metabolic rates as noted previously. This elevation in metabolic rates by leptin was markedly attenuated in mice that had been fed the low calcium (0.25%) diet. The physiological effects of leptin on oxygen consumption are seemingly dependent on dietary calcium concentrations. The molecular interactions between leptin and dietary calcium to elevate metabolic rates requires further investigation.