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Improved measurement of niacin in a variety of food samples by high performance liquid chromatography‐stable isotope dilution mass spectrometry
Author(s) -
Goldschmidt Robert J,
Wolf Wayne R
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a317-d
Subject(s) - niacin , chemistry , isotope dilution , chromatography , liquid chromatography–mass spectrometry , mass spectrometry , solid phase extraction , detection limit , sample preparation , selected reaction monitoring , tandem mass spectrometry , biochemistry
Analysis of the water‐soluble B‐vitamin, niacin, in food samples can be difficult due to interference of other components in the food matrix. With commercial availability of deuterium‐labeled nicotinic acid, we evaluated the use of stable isotope dilution mass spectrometry (IDMS) coupled with liquid chromatography (LC) to determine niacin in food samples. We developed an IDMS method based on the AOAC Peer‐Verified Method PVM1:2000 for niacin includes acid digestion, solid phase extraction (SPE) with a strong cation exchange (SCX) column, and reversed phase chromatography with a C18 column. Ionization is by positive ion electrospray. Although analysis can be accomplished in selected ion recording (SIR) mode, it is subject to interference problems similar to those found with other LC determinations of niacin.. The added selectivity of a multiple‐reaction monitoring (MRM) mode, which largely eliminated interference problems. The method has been applied to a variety of food samples, both fortified and those with only endogenous niacin, including some with sub‐ppm niacin levels. The method is capable of determining niacin levels even at the ppb range in a wide variety of food materials. Availability of accurate, highly precise IDMS methods to measure water‐soluble vitamins in foods will lead to better food composition data.