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Influence of extraction solvents and delipidation on assay of isoflavones from soybean
Author(s) -
Luthria Devanand L,
Biswas Ronita,
Natarajan Savithiry
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a317-a
Subject(s) - isoflavones , extraction (chemistry) , chromatography , chemistry , solvent , accelerated solvent extraction , sonication , high performance liquid chromatography , dimethyl sulfoxide , biochemistry , organic chemistry
Significant variation in the solvents and procedures used for the extraction of isoflavones from soybeans has been reported in peer‐reviewed literature by different research groups. This systematic study was undertaken to address the influence of delipidation and extraction solvents and techniques on the assay of isoflavones from soybean. Comparison of seven different solvent mixtures used for the extraction of isoflavones from ground soybean with and without lipids was carried out. Assessments of six commonly used extraction techniques (shaking, vortexing, sonication, stirring, Soxhlet, and pressurized liquid extraction (PLE)) with an optimized extraction solvent mixture was also performed. Both the total isoflavone content and the isoflavone HPLC profiles varied significantly with different extraction solvents and techniques. Optimum total isoflavone recoveries from soybean samples were obtained from delipidated soybeans with a dimethyl sulfoxide:ethanol:water (5:75:25, % v/v/v) solvent mixture using PLE. Precise quantitation of soy isoflavones will allow researchers to accurately determine the dietary intake levels and safety guidelines for potentially bioactive phytochemicals necessary to achieve the desired health‐beneficial effects. Supported by ARS/USDA and Dionex, Inc., USA.