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Alterations of the Classic Pathway of Complement in Adipose Tissue of Obesity and Insulin Resistance
Author(s) -
Zhang Jinhui,
Wright Wendy,
Bernlohr David A.,
Cushman Samuel W.,
Chen Xiaoli
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a294
Subject(s) - adipose tissue , insulin resistance , decorin , medicine , endocrinology , adipose tissue macrophages , white adipose tissue , inflammation , 3t3 l1 , biology , insulin , adipocyte , microbiology and biotechnology , proteoglycan , extracellular matrix
To investigate adipose expression of C1 complement subcomponents and C1 activation regulator decorin and its regulation in obesity and insulin resistance, we examined the levels of mRNAs for C1q, C1r, C1s and decorin and their regulation by TNF ¦Á and TZD in 3T3‐L1 adipocytes, primary adipose and S‐V cells, and adipose tissues from various genetic and diet‐induced obese models of rodents as well as insulin resistant humans. Quantitative real‐time RT‐PCR and cDNA microarrays were performed to detect changes in gene expression. C1q, C1r, C1s, and decorin were expressed in undifferentiated 3T3‐L1 preadipocytes at a relatively higher level, but expression declined upon full differentiation. TNF‐¦Á treatment significantly increased C1s, but decreased C1q expression in 3T3‐L1 adipocytes. Increased expression of C1q, C1r, C1s and decorin was observed in primary adipose, particularly S‐V cells isolated from obese Zucker rats. Up‐regulation of C1r and C1s expression was also perceived in adipose cells from insulin resistant humans and cultured rat adipose cells as they developed into insulin resistance. TZD administration to HF‐DIO mice led to the reversal of C1q and decorin expression. Our findings demonstrate that dysregulation of the classic pathway of complement occurs in obesity, suggesting its possible role in adipose tissue inflammation and insulin resistance.