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Role of C17orf37/MGC14832 in prostate cancer cell proliferation and migration
Author(s) -
Dasgupta Subhamoy,
Vishwanatha Jamboor K
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a289-b
Subject(s) - prostate cancer , gene knockdown , cancer cell , biology , immunoprecipitation , cancer research , transfection , cell growth , microbiology and biotechnology , cancer , cell migration , colocalization , cell culture , genetics
C17orf37/MGC14832 located on human chromosome 17q12 is activated during amplification of a core region at the ERBB2 . C17orf37 protein is expressed as a cytosolic protein in cancer cells. However the potential role and functional significance of C17orf37 over expression in cancer cells is not known. Here we hypothesize that C17orf37 is involved in prostate cancer cell migration and plays an important role in metastatic progression of the disease. RNAi mediated down‐regulation of C17orf37 from prostate cancer DU‐145 cells showed a reduction in cell migration. We also found that knockdown of C17orf37 mRNA in DU‐145 cells resulted in a reduction of ERBB2 messenger expression which in turn down regulated two isoforms of VEGF . Using an in vitro tumor invasion assay system we observed that C17orf37 down regulated DU‐145 cells show a reduction in the migration of cells compared to the untreated or mock transfected cells. By the yeast two hybrid interaction analysis we found that C17orf37 is an interactor of annexinA2, a Ca +2 dependent membrane binding protein highly over expressed in cancer cells and this interaction was validated by immunoprecipitation and colocalization studies. Currently we are investigating the functional significance of C17orf37‐anxA2 interaction and its potential role in cancer cell progression and proliferation. (Supported grants from the National Institutes of Health (CA109593 and MD 001633).