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TNF‐α signaling through NF‐κB in chromaffin cells‐identification of novel targets with suppression subtractive hybridization study
Author(s) -
AitAli D,
Turquier V,
Grumolato L,
Yon L,
Mounien L,
Jegou S,
Derambure C,
Salier JP,
Eiden L E,
Vaudry H,
Anouar Y
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a287-d
Subject(s) - microbiology and biotechnology , biology , tumor necrosis factor alpha , suppression subtractive hybridization , signal transduction , chromaffin cell , mapk/erk pathway , nfat , endocrinology , medicine , gene expression , transcription factor , adrenal medulla , gene , biochemistry , cdna library , catecholamine
Immune‐autonomic interactions important in immune and stress responses occur at the adrenal medulla. Cytokines affect chromaffin cell secretory function through neuroendocrine cell‐unique signaling pathways, which require further characterization. Thus, TNF‐α stimulates VIP, galanin and secretogranin II biosynthesis following long‐term (24–48 hr) stimulation of bovine adrenochromaffin cells through the type 2 TNF‐α receptor, and activation of ERK 1/2, p38, and AP‐1 transcription factor (Ait‐Ali et al., Mol. Endocrinol . 18 : , 2004), however we have now found that TNF‐α stimulation of neuropeptide gene expression also requires prostaglandin and calcium elevation, and activation of NF‐κB. Analysis of global gene expression changes in adrenochromaffin cells treated with TNF‐α, using suppression subtractive hybridization (SSH), allowed us to identify several additional TNF‐α‐stimulated genes implicated in NF‐kB signaling in other cell contexts, such as NF‐κB inhibitor (IκB), calcium modulating ligand (CAMLG), an integral membrane protein implicated in calcium/calcineurin activation, and butyrate‐induced transcript 1 and mitogen‐inducible gene‐6 (MIG‐6). Thus, TNF‐α signaling through the type 2 receptor may involve multiple components related to activation of NF‐κB, and likely relevant to the unusually prolonged action of TNF‐α on neuropeptide gene expression in chromaffin cells.