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Investigation of the function of transcriptional coactivator ADA2 in the growth, development, and stress response of Arabidopsis thaliana
Author(s) -
Gordon Hillary,
Hark Amy T.
Publication year - 2007
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.21.5.a286-b
Subject(s) - mutant , biology , arabidopsis thaliana , genetics , transgene , wild type , arabidopsis , phenotype , gene , coactivator , abiotic stress , microbiology and biotechnology , chromatin , transcription factor
Histone acetyltransferases (HATs) modify chromatin structure through the addition of acetyl groups. The HAT GCN5 requires transcriptional coactivator ADA2 for its function in vitro and in vivo. Arabidopsis thaliana contains two homologues of ADA2 ‐ ADA2a and ADA2b . The ADA2b gene can be mutated to induce known phenotypic changes; however, under normal growing conditions (25°C, 24 hr light) the ada2a mutant phenotype is identical to that of wildtype. Furthermore, overexpressing the ADA2a gene will not rescue an ada2b mutant. A series of transgenes are being constructed in which certain domains of the ADA2b gene are selected and combined with the complementary domains from the ADA2a gene. The transgenes will be incorporated into the ada2b mutant plant's genome and the phenotype and genotype of resulting transgenic offspring analyzed to determine if the transgene rescued the ada2b mutation. Assessing the effect of the transgenes will allow the ADA2b domains required for proper growth to be identified. Environment plays a role in the growth and development of A. thaliana and it is often used as a model in abiotic stress studies. It has been shown that ada2b mutant plants have a freezing resistance superior to wildtype, indicating ADA2b may play a complex role in stress response. In this study, wildtype and ada2a mutants have been subjected to waterlogging stress in hopes of revealing an ada2a phenotype.